Catalog # PM2291


PYK2 (C-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 116 kDa

Isotype IgG1

$245

Protein tyrosine kinase, Pyk2 (CAKb, RAFTK, CADTK), is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration of various cell types. In T-cells, Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR. Phosphorylation of Tyr-402 is required for the phosphorylation of other tyrosines in Pyk2 and provides a binding site for Fyn SH2 during T-cell activation.

 

Clone M229 was generated from a recombinant human PYK2 protein that included amino acids residues in the C-terminal region. This sequence is conserved in rat and mouse PYK2, and has low homology to other related kinases.

*For more information, see UniProt Accession Q14289
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 116 kDa* band corresponding to PYK2 on SDS-PAGE immunoblots of mouse brain tissue.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of mouse brain. The blot was probed with anti-PYK2 (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3), and 1:2000 (lane 4).



This kit contains:

KIT SUMMARY