Catalog # PM1441


p130 Cas (C-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
IHC1:250
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 130 kDa

Isotype IgG1

$245

p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Src binding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs). Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas central substrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.

 

References

Shin, N-Y. et al. (2004). J Biol Chem. 279(37):38331.
Kim, D.H. et al. (2003). Biochem Biophys Res Comm. 300:141.
Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
Nakamoto, T. et al. (1996) J Biol Chem. 271(15):8959.

Clone M144 was generated from a recombinant protein containing amino acid residues in the C-terminal region of rat p130 Cas. This rat sequence is highly conserved in human, mouse, and chicken p130 Cas.

*For more information, see UniProt Accession Q63767
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 130 kDa* protein corresponding to the molecular mass of p130 Cas on SDS-PAGE immunoblots of human A431, endothelial, and Hct116 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).



This kit contains:

KIT SUMMARY