Catalog # MP4561

Myosin 13/Extraocular Myosin (C-terminal region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 215 kDa

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

References

Moncman, C.L. et al. (2011) Invest Ophthalmol Vis Sci. 52(7):3962.
Briggs, M.M. & Schachat, F. (2002). J Exp. Biol. 205:3133.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.

Western blot analysis of MYH13 in mouse extraocular muscle. The blot was probed on the left with anti-MYH13/Extraocular myosin (Hinge region) antibody (MP4571) and on the right with anti-MYH13/Extraocular myosin (C-terminal region) antibody (MP4561).

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region from mouse MYH13/extraocular myosin. This sequence has 100% identity with rat and human MYH13, and has no homology to other myosin family members.

*For more information, see UniProt Accession B1AR69

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using MYH13 (C-terminal region) peptide (without carrier). The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH13 on SDS-PAGE immunoblots of mouse extraocular muscle, and does not detect MYHs in mouse diaphragm, gastrocnemius, or brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: