Catalog # MP3721

Memo (N-terminal region) Antibody

Rabbit Polyclonal

Application Dilution
ELISA1:2000
ICC1:200
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 33 kDa

DATASHEET  




$245

During cell migration, actin assembly drives cell membrane protrusion, while microtubules (MTs) extend within protrusions to promote adhesion site turnover. Memo (mediator of ErbB2-driven cell motility) is an effector of the ErbB2 receptor tyrosine kinase involved in breast carcinoma cell migration. This effector may be important for mediating ErbB2-regulated changes in actin and MT dynamics during cell motility. Memo, a 297-amino-acid protein, has homology to class III nonheme iron-dependent dioxygenases, however it has not been shown to display metal binding or enzymatic activity. It has been shown to bind ErbB2 (Tyr-1227) phosphopeptide via its putative enzymatic active site. Memo and PLCγ1 interaction with ErbB2 is essential for HRG-induced chemotaxis. Furthermore, organization of the lamellipodial actin network is coordinated by signaling from Memo to the RhoA–mDia1 pathway localized to the plasma membrane. In addition, Memo may regulate actin dynamics by promoting cofilin depolymerizing and severing of F-actin.

 

References
Meira, M. et al. (2009) J. Cell Sci. 122:787.
Qiu, C. et al. (2008) J. Biol. Chem. 283:2734.
Zaoui, K. et al. (2008) J. Cell Biol. 183:401.

Western blot analysis of Memo expression in adult mouse heart (lane 1 & 4), mouse C2C12 cells (lane 2 & 5), and rabbit spleen fibroblast cells (lane 3 & 6). The blot was probed with anti-Memo (N-terminal region) (MP3721; lanes 1-6) in the presence (lanes 4-6) or absence (lanes 1-3) of Memo blocking peptide (MX3725).

Immunocytochemical labeling of Memo in rabbit spleen fibroblasts that were fixed in paraformaldehyde and permeabilized with NP-40. The cells were probed with the Memo (N-terminal Region) MP3721, then the antibody was detected using goat anti-rabbit DyLight® 594. The antibody was used in the absence (left) or presence (right) of it's blocking peptide (MX3725).



Memo synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human Memo. This peptide sequence is highly conserved in rat, mouse, and chicken Memo.

*For more information, see UniProt Accession Q9Y316
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Memo (N-terminal region) peptide (without carrier). The antibody detects a significant 33 kDa* protein corresponding to the molecular mass of Memo on SDS-PAGE immunoblots of mouse heart, mouse C2C12, and rabbit spleen fibroblasts.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY