Catalog # MM0271

Mitofilin Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 90 kDa

Isotype IgG1

DATASHEET  




$295

Mitofilin is an important organizer of mitochondrial architecture. The mitofilin sequence encodes a polypeptide with a central alpha-helical region with predicted coiled coil domains flanked by globular amino and carboxy termini. There are four isoforms of mitofilin, and 90/91 kDa mitofilin forms have been observed in western blots. Mitofilin is located in the inner mitochondrial membrane and interacts with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. These mitofilin-containing hetero-oligomeric protein complexes form the mitochondrial inner membrane organizing system (MINOS). MINOS integrity is required for the structural maintenance of the inner mitochondrial membrane. This mitochondrial region contains cristae membranes that form large tubular invaginations that protrude into the mitochondrial matrix. These cristae membranes contain the enzyme complexes of the oxidative phosphorylation machinery. MINOS deficiency causes loss of crista junction structures and the detachment of cristae from the inner boundary membrane.

 

References
Zerbes RM, et al. (2012) Biol Chem. 393(11):1247.
Odgren PR, et al. (1996) J Cell Sci. 109(9):2253.

Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.

Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight® 594.



Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line.

*For more information, see UniProt Accession Q16891
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M027 detects a 90 kDa* band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates using MM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY