Catalog # IP1281

Integrin β4 (Tyr-1494), phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:4000
ICC	1:100
WB	1:2000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 200 kDa

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin α6β4receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the Integrin β4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly this site is critical for integrin α6β4 increases in carcinoma invasion.

References

Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.

Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-Integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin β4 (lanes 5 & 6).

Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.

Phospho-Integrin β4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human Integrin β4. This peptide sequence is found in all three Integrin β4 isoforms and is highly conserved in rat and mouse Integrin β4.

*For more information, see UniProt Accession P16144-2

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-Integrin β4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin β4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Dilly, AK et al. (2017) Exp Cell Res. 351(1):1-10. (WB: human A431 cells)
Coleman, D.T. et al. (2015) PLoS One. 10(5):e0125399 (WB: HCC-1806, MCF-10A)
Soung, Y. et al. (2013) BMC Cell Biol. 1:14. (WB: MDA-MB-231, MDA-MB-435)
Soung, Y. & Chung, J. (2011) Mol Cancer Ther 10(5):883. (WB: human A431, MDA-MB-231)
Yang, X. et al. (2010) Mol Cell Biol. 30(22):5306. (WB: Y1494F mutants)
Kim, H.I. et al. (2008) Cancer Prev Res. 1(5):385. (WB: human b4 transfectants)

This kit contains: