Catalog # IM3741


IκBα (Ser-32/Ser-36), phospho-specific

Mouse Monoclonal

Application Dilution
ELISA1:2000
IP1:100
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 38 kDa

Isotype IgG1

$245

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

 

References
Waris et al. (2003) J Biol Chem 278(42):40778.
Bui, N.T. et al. (2001) J Cell Biol 152(4):753.
Finco, T.S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:11884.
Clone 39A1413 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 32 and 36 of human IκBα. This peptide sequence is highly conserved in mouse, rat, dog, cow, and pig IκBα.

*For more information, see UniProt Accession P25963
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 0.5% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of Jurkat cells treated with calpain inhibitor (ALLN) followed by TNFα, but the antibody does not detect this band in untreated cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of Jurkat cells untreated (lanes 1 & 3) or treated with TNFα (1 nM). The blots were probed with anti-IκBα (lanes 1 & 2) or anti-IκBα (Ser-32/Ser-36) (lanes 3 & 4).



This kit contains:

KIT SUMMARY