Catalog # IM0411


Integrin β1 (Extracellular region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 130 kDa

Isotype IgG2a

$295

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin β1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.

 

References

Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.

Clone M041 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the NCI-H1915 lung cancer cell line.

*For more information, see UniProt Accession P05556
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M0411 detects 120-150 kDa* bands corresponding to Integrin β1 on SDS-PAGE immunoblots of native human A431, LNCaP, MCF7, and NCI-H446 cell lysates, as well as human lung tissue. The antibody does not detect the denatured form of Integrin β1. Clone M0411 can be used in western blot, immunocytochemistry, and immunoprecipitation, as well as for ELISA detection when paired with Integrin β1 IM0041 as the capture antibody.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of native cell lysates from human NCI-H446 lung cancer cells (lane 1), LNCaP prostate cancer cells (lane 2), and MCF7 breast cancer cells (lane 3). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0411) at 1:1000 dilution.

Immunocytochemical labeling of Integrin β1 in aldehyde fixed and NP40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (IM0411). The antibody was detected using goat anti-mouse Ig:DyLight® 594.



This kit contains:

KIT SUMMARY