Catalog # IM0041

Integrin β1 (Extracellular region) M004 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:100
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 130 kDa

Isotype IgG1

Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin β1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.

References

Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.

Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and LNCaP (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0041) at 1:1000 dilution.

Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (clone M004). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-integrin β1 (IM0041) for ELISA capture of human recombinant integrin β1 extracellular region. Capture was detected by mouse monoclonal anti-integrin β1 (IM0411) followed by appropriate secondary antibody conjugated to HRP.

Clone M004 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α expressed in the A431 epidermoid carcinoma cell line.

*For more information, see UniProt Accession P05556

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M004 detects 120-150 kDa* bands corresponding to Integrin β1 on SDS-PAGE immunoblots of native human A431, A549, MDA-MB-231, and LNCaP cell lysates. The antibody also detects native recombinant human Integrin α1/β1, α2/β1, and α3/β1 complexes, but does not detect recombinant human Integrin α6/β4 or αV/β3 complexes. The antibody does not detect the denatured form of Integrin β1. Clone M004 can be used in western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well for detecting live, unfixed cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: