Catalog # GM3311

Grb2 Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:50
IP1:50
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 24 kDa

Isotype IgG1

DATASHEET  




$325

Crk and Grb2 family adaptor proteins are involved in a variety of cell signaling pathways related to human diseases. These adaptors have Src homology 2 (SH2) and Src homology 3 (SH3) domains, which are docking sites for several signaling proteins, including receptors, kinases, and GTPase regulators. In addItion, other protein-protein interactions may also utilize Crk and Grb2 domain interactions to modulate cell signaling pathways. The DOCK family of proteins (DOCK3, DOCK4, and DOCK5) can interact with Crk and may be important Crk effector proteins. The cell cycle regulator p27Kip1 can interact with Grb2, and this implicates Grb2 activity in cell signaling pathways that alter cell cycle progression.

 

References

Feller, S.M. & Lewitzky, M. (2006) Curr. Pharm. Des. 12(5):529.
Feller, S.M. (2001) Oncogene. 20(44):6348.

Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Immunocytochemical labeling of Grb2 in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Grb2 (GM3311) antibody, then detected using appropriate secondary antibody conjugated to Cy3.



Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2.

*For more information, see UniProt Accession P62994
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY