Catalog # GM1321
GSK-3α/β (Tyr-279/Tyr-216), phospho-specific Antibody
Mouse Monoclonal
| ELISA | 1:2000 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 50 kDa
Isotype IgG1
Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (α and β) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3β plays a critical role in cell survival by phosphorylating nuclear factor-κB (NF-κB) p65 subunit, leading to NF-κB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3α), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of β-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3β activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3β (Serine 21 in GSK-3α). This phosphorylation may be involved in later phases of neuronal apoptosis.
References
Xinbo, L. et al. (2003) Mol Cancer Ther. 2:1215.
Takahashi-Yanaga, F. et al. (2004) Bioch Biophys Res Com. 316(2):411.
Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3β (lanes 1 & 2) or anti-GSK-3α/β (Y279/Y216) (lanes 3 & 4).
*For more information, see UniProt Accession P49841
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
Sui Y. et al. (2019) Brain Res. 1719:1. (WB: APP/PS1 double-transgenic mice)Zhu, C. et al. (2017) Mol Neurobiol. 54(10):7964. (WB: mouse hippocampal neurons)
Li, R. et al. (2015) Neuroscience. 21(294): 14. (WB: rat primary neurons)
Gloggin, S. L. et al. (2014) Toxicology reports 1: 5443. (WB: mouse mFC and HPC)
Azkona, G. et al. (2014) Neuropharmacology. 79:726. (WB: rat brain)
Ding, Y. et al. (2010) Neurobiol Disease. 39(2):156. (WB: mouse cerebral cortex)
Turnquist, H.R. et al. (2010) Blood. 115(23):4758. (WB: mouse dendrite cells)
Ding, Y. et al. (2008) J Neurosci. 28(45):11622. (WB: mouse cortex, hippocampus)
This kit contains:
