Catalog # GM1311

GSK-3β (N-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:1000
ICC1:50
IHC1:250
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 46 kDa

Isotype IgG1

DATASHEET  




$245

Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (α and β) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3β plays a critical role in cell survival by phosphorylating nuclear factor-κB (NF-κB) p65 subunit, leading to NF-κB transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3α), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of β-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3β activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3β (Serine 21 in GSK-3α). This phosphorylation may be involved in later phases of neuronal apoptosis.

 

References

Takahashi-Yanaga, F. et al. (2004) Bioch Biophys Res Com. 316(2):411.
Xinbo, L. et al. (2003) Mol Cancer Ther. 2:1215.
Hagen, T. et al. (2002) J Biol Chem. 277(26):23330.

Western blot analysis of a human recombinant GSK3β N-terminal fragment (lanes 1 & 2) and endogenous GSK3β expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3β at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).

Immunocytochemical labeling of GSK3β in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK3β (GM1311) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



Clone M131 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human GSK-3β. This sequence is highly conserved in rat and mouse GSK-3β, and has 65% homology to similar regions in GSK-3α.

*For more information, see UniProt Accession P49841
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This mouse monoclonal antibody was purified with protein A chromatography. The antibody detects a 46 kDa* protein corresponding to the apparent molecular mass of GSK-3β on SDS-PAGE immunoblots of rabbit fibroblasts, mouse brain, as well as human SKN-SH and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY