Catalog # GM0121

Girdin (C-terminus) Antibody

Mouse Monoclonal

Application / Dilution

ICC	1:50
IHC	1:50
IP	1:50
WB	1:250

Size 100 μl

Species Reactivity Hu

MW 250 kDa

Isotype IgG1

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

References

Hayano S. et al, (2015) J Mol Cell Cardiol. 88:55
Lin, C. (2011) Sci Signal. 4(192): ra64
Jiang, P. (2008) Cancer Res. 1;68(5):1310
Simpson, F. (2005) Traffic. 6(6):442

Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).

Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight® 594.

Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin.

*For more information, see UniProt Accession Q3V6T2

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M012 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: