Catalog # FM3151


Fascin (Ser-39), phospho-specific

Mouse Monoclonal

Application Dilution
ELISA1:1000
WB1:500
IHC1:100

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 55 kDa

Isotype IgG1

$295

Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.

 

References
Aratyn, Y.S. et al. (2007) Mol Biol Cell. 18(10):3928.
Kraft, R. et al. (2006) J Neurosci 26:8734.
Vignjevic, D. et al. (2006) J Cell Biol. 174(6):863.
Adams, J.C. (2004) Curr Opin Cell Biol. 16(5):590.
Clone M315 was generated from a phospho-Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3.

*For more information, see UniProt Accession Q16658
Mouse monoclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using phospho-fascin (Ser-39) peptide (without carrier). The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of mouse C2C12 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) mouse monoclonal (lanes 3 & 4).



This kit contains:

KIT SUMMARY