Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLCγ. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.
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Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14:1680.
Schlaepfer, D.D. et al. (1994) Nature 372:786-791.
*For more information, see UniProt Accession Q05397
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Stone, JD et al. (2016) J Non Invasive Vasc Invest. 1:2-9. (FC: rat ATr5 smooth muscle cells)
Oyanadel, C. et al. (2018) Mol Biol Cell. 29(5):557-574. (WB: MDCK cells)
Ma, SN et al. (2017) Evid Based Complement Altern Med. 2017:4535326. (WB: rabbit cartilage)
Abdel-Naby, W. et al. (2017) PLoS One. 12(11):e0188154. (ICC: rabbit cornea)
Creekmore, A.L. et al. (2013) Biomolecules 3(3): 386. (WB: mouse cells)
Teravainen, T. et al. (2013) PLoS ONE 8(8):e71485. (WB: MDCK cells)
Lin, S. & Mequanint, K. (2012) Biomaterials 33(29):7047. (WB: HCASMC)
Akama, K. et al. (2011) BBA Prot & Proteo. 1814(2):265. (WB: monkey neurons)
Kong, T. et al. (2009) Mol Biol Cell. 20:4596. (WB: canine MDCK cells)
This kit contains: