Catalog # FM0131
Fibronectin (Cell/Heparin2 Binding region) Antibody
Mouse Monoclonal
| ELISA (Cap) | 1:100 |
| ICC | 1:50 |
| IP | 1:50 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu
MW 250 kDa
Isotype IgG1
In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230 kDa to 270 kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.
References
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).
Western blot image of native human cell lysates: A549 (lanes 1 & 5), MeWo (lanes 2 & 6), and MDA-MB-231 (lanes 3 & 7), as well as human plasma fibronectin (lanes 4 & 8). The blot was probed with mouse monoclonal antibodies anti-fibronectin FM0141 (lanes 1-4) or anti-fibronectin FM0131 (lanes 5-8) at 1:1000.
Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M013). The antibody was detected using goat anti-mouse Ig DyLight® 594.
Representative Standard Curve using mouse monoclonal anti-fibronectin (FM0131) for ELISA capture of human recombinant fibronectin protein. Capture was detected by using anti-fibronectin (FM0221) biotin conjugate followed by streptavidin conjugated to HRP.
*For more information, see UniProt Accession P02751
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
