Catalog # FM0131

Fibronectin (Cell/Heparin2 Binding region) Antibody

Mouse Monoclonal

Application / Dilution
ELISA (Cap)1:100

Size 100 μl

Species Reactivity Hu

MW 250 kDa

Isotype IgG1



In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230 kDa to 270 kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.


Zollinger AJ & Smith ML (2017) Matrix Biol. 60-61:27.
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).

Western blot image of native human cell lysates: A549 (lanes 1 & 5), MeWo (lanes 2 & 6), and MDA-MB-231 (lanes 3 & 7), as well as human plasma fibronectin (lanes 4 & 8). The blot was probed with mouse monoclonal antibodies anti-fibronectin FM0141 (lanes 1-4) or anti-fibronectin FM0131 (lanes 5-8) at 1:1000.

Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M013). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-fibronectin (FM0131) for ELISA capture of human recombinant fibronectin protein. Capture was detected by using anti-fibronectin (FM0221) biotin conjugate followed by streptavidin conjugated to HRP.

Clone (M013) was generated from a proprietary antigen related to native human fibronectin from the A549 lung carcinoma cell line.

*For more information, see UniProt Accession P02751
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M013 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of native human A549, MeWo, and MDA-MB-231 cells, as well as human plasma fibronectin. The antibody does not detect the denatured form of fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: