Catalog # EP2821

Ephexin-1 (C-terminal region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:50
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 80 kDa

The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.

References

Zhang, Y. et al. (2007) J Biol Chem. 282(42):31103.
Sahin, M. et al. (2005) Neuron 46:191.
Shamah, S.M. et al. (2001) Cell 105:233.

Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Ephexin-1 (C-terminal region) (lanes 1 & 2) and anti-Ephexin-1 (Tyr-87) (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal Ephexin-1 (C-terminal region) and Ephexin-1 (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Ephexin-1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse ephexin-1. This sequence has significant homology to the same region in human and rat ephexin-1, and has low homology to other known proteins.

*For more information, see UniProt Accession Q8CHT1

Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This polyclonal antibody was affinity purified using ephexin-1 (C-terminal region) peptide. The purified antibody detects an 80 kDa* band corresponding to ephexin-1 in Western blots of human A431, Jurkat, and HUVEC cells, as well as rat PC12 cells and mouse brain tissue.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Fiore, L. et al. (2019) Exp Eye Res. 178:46. (WB/ICC: chick retinal neurons)
Rosas, O.R. (2014) Neural regeneration research 9(24): 2164. (WB: rat muscle)
Muto, A. et al. (2011) J Exp Med. 208(3):561. (WB: COS cells)
Rosas, O.R. et al. (2011) Dev Neurobiol. 71(7):595. (WB, IHC: rat spinal cord)

This kit contains: