Catalog # DP4471


mDia1 (a.a. 66-77)

Rabbit Polyclonal

Application Dilution
ELISA1:2000
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 140 kDa

$295

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia1 protein is activated by Rho and leads to ROCK-dependent stress fiber formation. Rho-activated mDia1 regulates serum response factor-dependent transcription. In cancers, mDia1 has been implicated in ras-mediated transformation, metastasis, and invasion. Thus, mDia1 is a Rho-activated formin with both cytoskeletal- and transcription-reguatling activities.

 

References
Narumiya, S. et al. (2009) Cancer Metastasis Rev. 28:65.
Ishizaki, T. et al. (2001) Nat Cell Biol. 3:8-14.
Watanabe, N. et al. (1999) Nat Cell Biol. 1:136-143.
mDia1 synthetic peptide corresponding to amino acids 66-77 in the N-terminal region of human mDia1 (Diap1). This peptide sequence is highly conserved in rat and mouse mDia1, and has low homology to other formins.

*For more information, see UniProt Accession O60610
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using mDia1 (a.a. 66-77) peptide (without carrier). The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of mDia1 on SDS-PAGE immunoblots of human K562, human Jurkat, and mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).



This kit contains:

KIT SUMMARY