Catalog # DM3511

DAAM1 (N-terminal region) Antibody

Mouse Monoclonal

Application Dilution
ELISA1:1000
ICC1:200
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 125 kDa

Isotype IgG2a

DATASHEET  




$245

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Dishevelled associated activator of morphogenesis proteins (DAAM1 and DAAM2) are formin family members involved in WNT signaling. DAAM1 is ubiquitously expressed and may be important for regulating actin dynamics in several cell types. DAAM1 can bind RhoGTPase and dishevelled in WNT signaling pathways, and interacts with the SH3 domains of cell signaling mediators, such as c-Src.

 

References

Aspenstrom, P. et al. (2006) Exp Cell Res. 312(12):2180.
Kida, Y. et al. (2004) Brain Res Dev Brain Res. 153(1):143.
Habas, R. et al. (2001) Cell 107(7):843.

Western blot analysis of DAAM1 expression in mouse C2C12 (lane 1), human A431 (lane 2), and K562 (lane 3) cell lysates. The blots were probed with mouse monoclonal DAAM1 (N-terminal region) antibody at 1:500.

Immunocytochemical labeling of DAAM1 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal DAAM1 (N-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The corresponding phase image is shown to the right.



Clone 5D3 was generated from a recombinant protein that included amino acid residues 1 to 111 in human DAAM1. This sequence has less than 50% homology to the N-terminal region of DAAM2.

*For more information, see UniProt Accession Q9Y4D1
Mouse monoclonal antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 125 kDa* protein corresponding to the apparent molecular mass of DAAM1 on SDS-PAGE immunoblots of human A431, K562, and mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY