Catalog # CP4021

β-Catenin (Tyr-654)[γ-Catenin (Tyr-644)], phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, Fr

MW 92 kDa



β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86, 489, and 654 in β-Catenin. Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin, while c-Abl phosphorylation of Tyr-489 decreases β-Catenin binding to N-Cadherin and leads to nuclear translocation and transcriptional activation.



Rhee, J. et al. (2007) Nat. Cell Biol. 9(8):883.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-β-Catenin (CM1181) (lanes 1 & 2), anti-β-Catenin (Tyr-654) (CP4021) (lanes 3 & 4), or anti-γ-Catenin (CM1111) (lanes 5 & 6).

Immunocytochemical labeling of phosphorylated β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Phospho-β-Catenin (Tyr-654) synthetic peptide corresponds to amino acid residues around tyrosine 654 of human β-Catenin. This peptide sequence is highly conserved in rat, mouse, chicken, and frog β-Catenins, and has high homology to the conserved site in γ-Catenin (Tyr-644).

*For more information, see UniProt Accession P35222
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to phospho-β-Catenin (Tyr-86) peptide before affinity purification using phospho-β-Catenin (Tyr-654) peptide (without carrier). The antibody detects 84 and 88kDa* proteins corresponding to the molecular mass of γ-Catenin and β-Catenin, respectively, on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but does not detect these proteins in control cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: