Catalog # CP3051


β-Catenin (Tyr-333)[γ-Catenin (Tyr-324)], phospho-specific

Rabbit Polyclonal

Application Dilution
ELISA1:1000
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck, F

MW 92 kDa

$295

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86, 333, 489, and 654 in β-Catenin. Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin, while c-Abl phosphorylation of Tyr-489 decreases β-Catenin binding to N-Cadherin and leads to nuclear translocation and transcriptional activation. In addition, Tyr-333 phosphorylation is required for binding to the metabolic kinase, PKM2,and promotes nuclear accumulation of both proteins in gliomas.

 

References
Yang, W. et al. (2011) Nature. 480(7375):118-22.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.

Phospho-β-Catenin (Tyr-333) synthetic peptide (coupled to KLH) that includes amino acid residues around tyrosine 333 of human β-Catenin. This peptide sequence is highly conserved in rat and mouse β-Catenin, and has high homology to the conserved site in γ-Catenin (Tyr-324).

*For more information, see UniProt Accession P35222
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using phospho-β-Catenin (Tyr-333) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of β-Catenin on SDS-PAGE immunoblots of human A431 cells treated with pervanadate, but not in control cells. In addition, the pervanadate induced reactivity is not observed after alkaline phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1 & 3) then treated with akaline phosphatase (lanes 2 & 4). The blot was probed with CP1061 anti-β-Catenin (CP1061) (lanes 1 & 2) and CP3051 anti-β-Catenin (Tyr-333) (lanes 3 & 4).



This kit contains:

KIT SUMMARY