Catalog # CP2191


CRMP2 (Ser-522), phospho-specific

Rabbit Polyclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 70 kDa

$295

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.

 

References

Arimura, N. et al. (2000) J. Biol. Chem. 275(31):23973.
Uchida, Y. et al. (2005) Genes to Cells 10:165.
Cole, A.R. et al. (2006) J. Biol. Chem. 281(24):16591.
Mimura, F. et al. (2006) J. Biol. Chem. 281(23):15970.

Phospho-CRMP2 (Ser-522) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-522 in human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has homology to the conserved site in CRMP1 and CRMP4, but is not conserved in CRMP3 and CRMP5.

*For more information, see UniProt Accession Q16555
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using CRMP2 (Ser-522) peptide (without carrier). The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human mouse brain, and this band is removed after lambda phosphatase treatment. In addition, this reactivity can be specifically blocked using phospho-CRMP2 (Ser-522) peptide (CX2195).

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Moutal, A. et al. (2019) Neurobiol Pain. 5. pii: 100022. (WB: rat spinal cord)
Togashi, K. et al. (2018) Genes Cells. Oct. 29 epub. (IHC: mouse brain)
Moutal, A. et al. (2018) Mol Neurobiol. 55(5):4403. (WB/IHC: mouse brain)
Watamura, N. et al. (2016) J Neurosci Res. 94(1):15 (WB: mouse brain )
Sebastian, Q. et al. (2016) AIDS Res Hum Retrovir. 32(1):68 (WB: human peripheral blood mononuclear cells)
Moutal, A. et al. (2015) Mol Neurobiol. :1 (WB: rat primary cortical neurons)
Francois-Moutal L. et al. (2015) Pain. 156(7):1247. (WB: rat DRG neurons)
Wilson, S.M. et al. (2014) Front Cell Neurosci. 24(8):196 (WB: rat cortical neurons)
Wilson, S.M. et al. (2014) Front Cell Neurosci. 28(8):135 (WB: rat cortical neurons)
Qin, Z. et al. (2013) PLoS ONE 8(11):e80758. (WB: mouse brain )
Akama, K. et al. (2013) Biochim Biophys Acta. 1834(2):601. (WB: monkey ES, NSS, NS, and AstP)
Yonedaa, A. et al. (2012) Mol Cell Biol. 32(10):1788. (WB: rat fibroblasts, MDA-MB-231, MCF7, SW480, MDCK, SW620)
Namekata, H. et al. (2012) J Neurosci. 32(1):264. (WB: COS7)
Lim, Y-W et al. (2010) Bioch Biophys Res Comm 395(2):207. (WB: rat cortical neurons)

Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).

Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



This kit contains:

KIT SUMMARY