Catalog # CP2191
CRMP2 (Ser-522), phospho-specific Antibody
Rabbit Polyclonal
| ELISA | 1:2000 |
| ICC | 1:100 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 70 kDa
CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518,Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding.
References
Arimura, N. et al. (2000) J. Biol. Chem. 275(31):23973.
Uchida, Y. et al. (2005) Genes to Cells 10:165.
Cole, A.R. et al. (2006) J. Biol. Chem. 281(24):16591.
Mimura, F. et al. (2006) J. Biol. Chem. 281(23):15970.
Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).
Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
*For more information, see UniProt Accession Q16555
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
Moutal, A. et al. (2019) Neurobiol Pain. 5. pii: 100022. (WB: rat spinal cord)Togashi, K. et al. (2018) Genes Cells. Oct. 29 epub. (IHC: mouse brain)
Moutal, A. et al. (2018) Mol Neurobiol. 55(5):4403. (WB/IHC: mouse brain)
Watamura, N. et al. (2016) J Neurosci Res. 94(1):15 (WB: mouse brain )
Sebastian, Q. et al. (2016) AIDS Res Hum Retrovir. 32(1):68 (WB: human peripheral blood mononuclear cells)
Moutal, A. et al. (2015) Mol Neurobiol. :1 (WB: rat primary cortical neurons)
Francois-Moutal L. et al. (2015) Pain. 156(7):1247. (WB: rat DRG neurons)
Wilson, S.M. et al. (2014) Front Cell Neurosci. 24(8):196 (WB: rat cortical neurons)
Wilson, S.M. et al. (2014) Front Cell Neurosci. 28(8):135 (WB: rat cortical neurons)
Qin, Z. et al. (2013) PLoS ONE 8(11):e80758. (WB: mouse brain )
Akama, K. et al. (2013) Biochim Biophys Acta. 1834(2):601. (WB: monkey ES, NSS, NS, and AstP)
Yonedaa, A. et al. (2012) Mol Cell Biol. 32(10):1788. (WB: rat fibroblasts, MDA-MB-231, MCF7, SW480, MDCK, SW620)
Namekata, H. et al. (2012) J Neurosci. 32(1):264. (WB: COS7)
Lim, Y-W et al. (2010) Bioch Biophys Res Comm 395(2):207. (WB: rat cortical neurons)
This kit contains:
