Catalog # CP1061
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 92 kDa
β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C-terminal armadillo repeat of β-Catenin. This phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
*For more information, see UniProt Accession P35222
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Kinoshita-Kikuta, E. et al. (2014) J Electrophoresis. 58(1) (WB: SW480 and HEK293)
Vanderplanck, C. et al. (2018) Skelet Muscle. 8:2. (WB: mouse C2C12)
Beard, R.S. et al. (2011) Blood. 118(7):2007. (WB, ICC: mouse microvascular ECs)
Harris, E.S. & Nelson, W.J. (2010) Mol Biol Cell. 21:2611. (WB: HUVECs)
This kit contains: