Catalog # CM4911


Caspase-3 (p17 subunit)

Mouse Monoclonal

Application Dilution
IHC1:50
WB1:250

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 32 kDa

Isotype IgG1

$245

The caspases are a group of cysteine enzymes which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

 

References

Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13.
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.

Clone 31A1067 was developed against full-length recombinant human Caspase-3. The antibody recognizes an epitope in the large domain subunit (p17) of Caspase-3, and it will recognize pro Caspase-3 and the p17 cleavage fragment.

*For more information, see UniProt Accession P42574
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 0.05% BSA, and 0.05% sodium azide. Store at 4°C. For long term storage, store at –20°C.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects pro Caspase-3 at 32 kDa* and the large subunit of the cleaved form of Caspase-3 at 14-21 kDa. The antibody can be used for western blot, immunohistochemistry and immuno-electron microscopy.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of Caspase-3 in multiple human tissues using CM4911 at 1:100. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in human, mouse and rat heart lysate, respectively.



This kit contains:

KIT SUMMARY