Catalog # CM4301

Cdc42 M430 Antibody

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:200
WB1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 21 kDa

Isotype IgM

DATASHEET  




$245

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

 

References

Erickson, J.W. & Cerione, R.A. (2001) Curr. Opin. Cell. Biol. 13(2):153.
Munemitsu, S. et al. (1990) Mol. Cell. Biol. 10:5977.
Shinjo, K. et al. (1990) PNAS (USA) 87:9853.

Western blot analysis of endogenous Cdc42 in human jurkat cells (lane 1) and mouse brain (lane 2), as well as detection of a 52 kDa rat Cdc42 full length recombinant fusion protein (lane 3). The blots were probed with anti-Cdc42 antibody (CM4301).

Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.



Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.

*For more information, see UniProt Accession Q8CFN2
Mouse monoclonal antibody purified with affinity chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY