Catalog # CM2261


Cdk1 (N-terminal region)

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
WB1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 34 kDa

Isotype IgG1

$245

Cyclin-dependent kinases (Cdks) are a family of serine/threonine kinases that require association with regulatory subunits known as cyclins for activation. In addition, post-translational phosphorylation and dephosphorylation events regulate Cdk activity. Phosphorylation of Thr-160 in the T loop by Cdk-activating kinase (CAK) is an obligatory step in kinase activation. By contrast, phosphorylation of the Thr-14 and Tyr-15 residues by the Wee1 family of dual specificity kinases is inhibitory for the Cdks, and dephosphorylation of these residues by the Cdc25 family of phosphatases coincides with Cdk activation. Alternatively, Cdk5 appears to require different mechanisms for activation. This Cdk is activated through association with specific activators, including p35, p39, and p67. Cdk5 is primarily activated in neuronal cells, and only c-Abl kinase, rather than Wee family members, have been shown to phosphorylate Tyr-15 to regulate its activity.

 

References
Lee, J.H. et al. (2008) J Biol. Chem. May 19 epub.
Zukerberg, L. R. (2000) Neuron 26:633.
Poon, R.Y.C. et al. (1997) J Biol. Chem. 272( 9):5703.
Clone M226 was generated from a recombinant human Cdk1 protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat Cdk1, and has low homology to other Cdk family members.

*For more information, see UniProt Accession P06493
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects a 34 kDa* band corresponding to Cdk1 on SDS-PAGE immunoblots of human SYF and HeLa cells. The antibody does not detect other Cdk family members, such as Cdk2 and Cdk5.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human SYF fibroblasts before (lanes 1 & 3) and after (lanes 2 & 4) treatment with alkaline phosphatase. The blots were probed with anti-Cdk1 (N-terminal region) antibody (lanes 1 & 2) or anti-Cdk1 (Tyr-15) phospho-specific antibody (lanes 3 & 4).



This kit contains:

KIT SUMMARY