Catalog # CM0191


Cyclophilin B

Mouse Monoclonal

Application Dilution
ELISA1:2000
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 20 kDa

Isotype IgG1

$295

Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPI) that are targets of the immunosuppressant drug cyclosporin A. These isomerases have been proposed to aid in protein folding. Cyclophilin B (PPIB) is an ER-localized chaperone protein that associates with type I procollagen, and facilitates procollagen sorting and transport through the secretory compartment. Mutations in the cyclophilin B gene lead to aberrant biosynthesis of type I procollagen, and osteogenesis imperfecta, a disorder characterized by bone fragility. Cyclophilin B has also been implicated as a marker in several cancers, including glioblastomas, neuroblastomas, and gastric cancers. In gastric cancer, Stat3 suppression of miR-520d-5p leads to upregulation of its downstream target cyclophilin B, and enables the growth and survival of these cancer cells.

 

References

Li, T et al. (2017) Cancer Res. 77(5):1227.
Pyott, SM et al. (2011) Hum Mol Genet. 20:1595 .

Clone M019 was generated from a proprietary antigen related to cyclophilin B from the MCF7 breast cancer cell line.

*For more information, see UniProt Accession P23284
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M019 detects a 20 kDa* band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A. The antibody can be used for western blot, immunoprecipitation, protein ELISA, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (CM0191) at 1:500.

Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (CM0191). The antibody was detected using goat anti-mouse DyLight® 594.



This kit contains:

KIT SUMMARY