Catalog # CM0111


CD59 (glycoprotein)

Mouse Monoclonal

Application Dilution
ELISA (Cap)1:100
ICC1:100
IP1:100
WB1:1000

Size 100 μl

Species Reactivity Hu

MW 15 kDa

Isotype IgG1

$295

CD59 is a GPI-anchored membrane protein that is an inhibitor of the complement membrane attack complex (MAC). CD59 binds to complement components C8 and C9, preventing C9 polymerization and insertion into membranes. Rare cases of CD59 deficiency have been reported to cause paroxysmal nocturnal hemoglobinuria in human patients. Expression of CD59 on tumor cells and viral infected cells makes them resist antibody-dependent complement-mediated lysis. Inhibitors of CD59 expression or activity may suppress tumor cell resistance to complement-mediated attack, and these technologies have been actively pursued for therapeutic applications. In addition, CD59 may regulate insulin secretion by modulating exocytosis, and a glycated form of CD59 with no MAC inhibitory activity is found in diabetic patients.

 

References
Mevorach, D (2015) Mol Immunol. 67:51.
Ghosh P et al. (2015) Endocr Rev. 36(3):272.
Sugita, Y & Masuho, Y (1995) Immunotechnology 1:157.
Clone M011 was generated from a proprietary antigen related to the mature form of human CD59 from the MDA-MB-231 breast carcinoma cell line.

*For more information, see UniProt Accession P13987
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M011 detects 15-20 kDa* bands corresponding to CD59 on SDS-PAGE immunoblots of native human MDA-MB-231, A549, A431, and MCF7 cell lysates, as well as recombinant human CD59 protein. The antibody does not detect denatured CD59. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live, unfixed A549 and MeWo cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of recombinant human CD59 protein (lanes 1 & 5), A431 (lanes 2 & 6), A549 (lanes 3 & 7), and MCF7 (lanes 4 & 8) whole cell lysates under native conditions. The blots were probed with mouse monoclonal anti-CD59 (CM0111) at 1:1000 (lanes 1-4) and anti-CD59 (CM0151) at 1:1000 (lanes 5-8).

Immunocytochemical labeling of CD59 in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-CD59 (clone M011). The antibody was detected using goat anti-mouse Ig DyLight® 594.



Representative Standard Curve using mouse monoclonal anti-CD59 (CM0111) for ELISA capture of human recombinant CD59 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

This kit contains:

KIT SUMMARY