Catalog # AP5631
Asc (Tyr-144), phospho-specific
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 22 kDa
Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. Asc can be phosphorylated at Tyr-144 in a Syk and JNK-dependent manner. This phosphorylation is critical for Asc speck formation and Caspase-1 activation.
Roberts, T.L. et al. (2009). Science. 323(5917):1057.
Bürckstümmer, T. et al. (2009). Nat Immunol. 10(3):266.
*For more information, see UniProt Accession Q9EPB4
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:Yu, SH et al. (2019) J Ethnopharmacol. 239:111917. (WB: mouse bone marrow macrophages)
Dubois, EC et al. (2019) PLoS Pathog. 15(4):e1007709. (WB: mouse macrophage)
Furuya, MY et al. (2018) Arthritis Res Ther. 20(1):196. (WB: human neutrophils)
Kwak, SB et al. (2018) Mediators Inflamm. 2018:6054069. (WB: mouse J774A.1, BMDMs)
Hoyt, L.R. et al. (2016) J Immunol. 197:1322. (WB: mouse macrophage, LPS)
This kit contains: