Catalog # AP3871
Arp2 (Thr-237/Thr-238), phospho-specific Antibody
Rabbit Polyclonal
| ELISA | 1:2000 |
| ICC | 1:100 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms, Ck
MW 44 kDa
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.
References
Soderling, S.H. (2009). Sci Signal. 2(55):pe5 (Review).
LeClaire, L.L. et al (2008). J Cell Biol. 182(4):647.
Kelleher, J.F. et al. (1995). J Cell Biol. 131(2):385.
Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with anti-Arp2 (C-terminal) (lanes 1 & 2) or anti-Arp2 (Thr-237/Thr-238) (lanes 3 & 4).
Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
*For more information, see UniProt Accession P61160
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
Morov, AR et al. (2016) Open Biol. Jun;6(6) pii:160062. (IHC: amphioxus)Lauterborn, J.C. et al. (2017) Cereb Cortex. 27(4):2640. (WB: rat hippocampus)
Machlus, K.R. et al. (2016) Blood. 127(11):1468. (WB: mouse megakaryocytes)
Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 (WB: mouse macrophages)
Park, M. et al. (2013) J Biol Chem 288:33324. (WB: human brain microvascular endothelial cells hCMEC/D3)
Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. (WB: bovine aortic endothelial cells)
This kit contains:
