Catalog # PM3801

Phosphoserine/threonine M380 Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:1000
ICC	1:50
IP	1:50
WB	1:500

Size 100 μl

Species Reactivity Hu, Rt, Ms

Isotype IgG1

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

References

Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.
Hunter T.(1987) Cell. 50(6):823.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).

Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein).

Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Su, KH et al. (2019) Molecular Cell 76:116. (WB: human HEK293 cells)
Malleske, DT et al. (2018) Stem Cells. 36(12):1905. (WB: human Epithelial cells)
Xiao, N. et al. (2019) FASEB J. 33(1):163-174. (IP: Hek293 cells)
Su, KH et al. (2016) Nat Cell Biol. 18(5):527. (WB: human HEK293 cells)
Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 (WB: mouse macrophages)
Fester, L. et al. (2015) JSBMB S0960-0760(15)30109. (WB: Hippocampal neurons)
Paul, NR et al. (2015) J Cell Biol. 210(6):1013. (WB: human ovarian cancer cells)
Morinaga, J. et al. (2013) AJP Renal Phys. 305(2)F173. (WB: Normal rat Kidney Fibroblast)

This kit contains: