Catalog # CM0261

CD99 (Extracellular region) M026 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:100
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 30/32 kDa

Isotype IgG1

The glycosylated transmembrane protein CD99 is involved in many essential cellular functions including cell adhesion, migration, cell death, differentiation, and intracellular protein trafficking. The CD99 gene encodes two distinct proteins, type I is 32 kDa and type II is 28 kDa, which are a result of the alternative splicing of the cytoplasmic region. These CD99 isoforms are expressed in a cell-type-specific manner and may have distinct functions. CD99 is overexpressed in several types of sarcomas, lymphomas, gliomas, neuroendocrine tumors, and some breast cancers. In these tumors, CD99 may have oncogenetic functions that promote migration, invasion, and metastasis of tumor cells. However, other neoplasms, carcinomas, and sarcomas have CD99 expression in benign or early-stage tumors, but lower expression in the advanced-stage counterparts. In these tumors, CD99 may have oncosuppressor signaling, and its re-expression can lead to the reversal of malignancy. Thus, CD99 is an important membrane protein involved in many aspects of cell migration and adhesion in normal and diseased cells.

References

Manara MC, et al. (2018) Genes (Basel). 9(3) pii: E159.
Pasello M, et al. (2018) J Cell Commun Signal. 12(1):55.

Western blot analysis of human cell lysates: A549 (lane 1), A431 (lane 2), LNCaP (lane 3), MDA-MB-231 (lane 4) and MeWo (lane 5). The blot was probed with mouse monoclonal anti-CD99 (CM0261) at 1:1000.

Immunocytochemical labeling of CD99 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD99 (clone M026). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD99 (CM0261) for ELISA capture of human recombinant CD99 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Clone M026 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line.

*For more information, see UniProt Accession P14209

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M026 detects 30 and 32 kDa* bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation. In addition, the antibody labels live, unfixed MeWo cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: