Catalog # EM0421

EpCAM (Extracellular region) M042 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:50
IP	1:50
WB	1:500

Size 100 μl

Species Reactivity Hu

MW 35-45 kDa

Isotype IgG1

Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that has an extracellular domain with one thyroglobulin type-1 domain and a short cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium. Defects in EpCAM cause hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cell proliferation and differentiation; it up-regulates the expression of FABP5, MYC, and Cyclin A & Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells and in many types of epithelial carcinomas.

References

Armstrong, A. & Eck, S.L. (2003) Cancer Biol Ther. 2:320.
Baeuerle, P.A. & Gires, O. (2007) Br J Cancer. 96:417.

Western blot of native lysates including human EpCAM extracellular region (lane 1 & 6), MCF7 breast carcinoma (lane 2 & 7), A431 skin adenocarcinoma (lane 3 & 8), NCI-H1915 lung carcinoma (lane 4 & 9), and NCI-H446 lung carcinoma (lane 5 & 10). The blot was probed with mouse monoclonal anti-EpCAM (EM0391) (lanes 1-5) and mouse monoclonal anti-EpCAM (EM0421) (lanes 6-10) at 1:1000 each.

Immunocytochemical labeling of EpCAM in aldehyde fixed human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-EpCAM (EM0421). The antibody was detected using goat anti-mouse Ig:DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-EpCAM (EM0421) for ELISA capture of human recombinant EpCAM extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Clone (M042) was generated from the extracellular region of human EpCAM.

*For more information, see UniProt Accession P16422

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M042 mouse monoclonal antibody detects a 35-45 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, H1915, and MCF7 carcinomas. This antibody does not detect denatured EpCAM. The antibody works for native western blot, immunoprecipitation, ELISA, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: