Catalog # AM0081

β-Actin M008 Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:1000
ICC	1:50
IP-MS	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 42 kDa

Isotype IgG2b

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

References

Jungbluth, A. et al. (1995) FEBS Let. 375:87.

Baba, T. et al. (2003) J. Immunol. 170: 3762.

Winder, S.J. et al. (2005) J. Cell Sci. 118:651.

Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.

Western blot analysis of human HUVEC-CS (lane 1), rabbit spleen fibroblast (lane 2), human Jurkat (lane 3), human LNCaP (lane 4), human HeLa (lane 5), and mouse F9 (lane 6) cell lysates. The blot was probed with mouse monoclonal anti-β-Actin (AM0081) at 1:1000 (lanes 1-6).

Immunocytochemical labeling of β-Actin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-β-Actin (clone M008). The antibody was detected using goat anti-mouse DyLight® 594.

Clone M008 was generated from a proprietary antigen related to human β-actin in MDA-MB-231 breast cancer cell line.

*For more information, see UniProt Accession P60709

Mouse monoclonal, protein G purified antibody is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M008 detects a 42 kDa* protein corresponding to the molecular mass of β-actin on SDS-PAGE immunoblots of human cancer cell lines, as well as human recombinant β-actin. This actin antibody preferentially detects human β-actin with only weak reactivity toward actins in rat, mouse, or rabbit. The antibody works in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation. In addition, mass spectrometry analysis of immunoprecipitates using AM0081 in human A431 cell lysate confirmed that this antibody detects β-actin.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: