Catalog # AM1011
Akt (N-terminal region) Antibody
Mouse Monoclonal
| ELISA | 1:2000 |
| ICC | 1:250 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms
MW 60 kDa
Isotype IgG1
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.
References
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
Western blot analysis of A431 cells, serum starved overnight (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min. (lanes 2 & 4). The blot was probed with anti-Akt (Thr-34) (lanes 1 & 2) or anti-Akt1 (N-terminal region) (lanes 3 & 4).
Western blot analysis of A431 cells untreated (lanes 1 & 3) or treated with 100 ng/ml EGF for 60 min. (lanes 2 & 4). The blots were probed with monoclonal anti-phospho-Akt (Ser-473) (lanes 1 & 2) and monoclonal anti-Akt1 (N-terminal region) (lanes 3 &4).
*For more information, see UniProt Accession P31749
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
Wei, H. & Vander Heide, R. (2010) AJP Heart Circ. 298:H152. (WB: rat myocardium)Li, W. et al. (2009) Am J Physiol Cell Physiol (2009) 297:706. (WB: mouse C2C12 cells)
Chambers, M.A. et al. (2009) J Physiol. 587:3363. (WB: mouse muscle, C2C12)
Moylan, J.S. et al. (2008) AJP Cell. Phys. 295:986. (WB: mouse C2C12)
Smith, M. et al. (2007) Am J Physiol Cell Physiol 293:1947. (WB: mouse C2C12 cells)
This kit contains:
