Catalog # CM0331

CD55 (Extracellular region) M033 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:100
IP	1:50
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 80 kDa

Isotype IgG1

CD55, also known as Decay-Accelerating Factor (DAF) is an inhibitor of the complement system, and is broadly expressed in malignant tumors. In cancer, CD55 has been implicated in tumorigenesis, neoangiogenesis, and metastasis. CD55 may decrease complement mediated tumor cell lysis, inhibit tumor apoptosis, and promote invasive cancer cell motility. These roles in cancer may involve binding to the seven-span transmembrane receptor CD97. In neuroblastoma cells, CD55 contributes to growth of colonies and to invasion of cells, but not to stemness. In neuroblastoma cells, CD55 is upregulated in a small population of cells that are HIF-2α positive. This CD55-positive subpopulation is highly invasive and has low adhesion to fibronectin and collagen. In addition, CD55 expression correlates with poor prognosis in neuroblastoma patients.

References

Cimmino F et al. (2016) Oncogenesis 5:e212.
Mikesch JH et al.(2006) Cell Oncol. 28(5-6):223.

Native western blot analysis of human MDA-MB-231 breast cancer cell lysate (lanes 1 & 2),human recombinant CD55 extracellular region protein (lane 3), and CD44 extracellular region protein (lane 4). The blots were probed with mouse monoclonal anti-CD55 (CM0331) at 1:1000 (lane 1) and 1:4000 (lanes 2, 3, and 4).

Immunocytochemical labeling of CD55 in paraformaldehyde fixed human MDA-MB-231 breast cancer cells. The cells were labeled with mouse monoclonal anti-CD55 (CM0331). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD55 (CM0331) for ELISA capture of human recombinant CD55 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Clone M033 was generated from a proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.

*For more information, see UniProt Accession P08174

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M033 was purified using Protein G chromatography. The antibody detects 75-100 kDa* bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of "native" A431, HeLa, MCF7, and MDA-MB-231 cell lysates, but does not detect the denatured form of CD55. The antibody also detects a "native" human recombinant CD55 protein that includes the extracellular region. The antibody can be used for native western blot, immunoprecipitation, protein ELISA, and immunocytochemistry, as well for detecting CD55 in live, unfixed cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: