Catalog # CM0241

CD44 (Extracellular region) M024 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:100
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 80 kDa

Isotype IgG2a

Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. Ligands for CD44 include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. Overexpression of CD44 has been observed in many types of cancer, and the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.

References

Cichy, J., et al. (2003) J Cell Biol. 161(5):839
Goodinson, S., et al. (1999) Mol Path. 52(4):189
Tsukita, S., et al. (1994) J Cell Biol. 126(2):391

Western blot analysis of human A549 (lane 1), MDA-MB-231 (lane 2), and MeWo (lane 3) cell lysates, as well as human breast (lane 4), lung (lane 5), skin (lane 6), and brain (lane 7) tissue lysates. The blot was probed with mouse monoclonal anti-CD44 (CM0241) at 1:2000.

Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M024). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD44 (CM0241) for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Clone M024 was generated from a proprietary antigen related to the extracellular region of human CD44 expressed in the MeWo melanoma cell line.

*For more information, see UniProt Accession P16070

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M024 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of native human MDA-MB-231, A549, MeWo, and A431 cell lysates. The antibody also detects denatured CD44 but with lower affinity. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live unfixed MeWo cells, so the antibody may be useful for isolating CD44+ cells and for CD44 functional assays.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: