Catalog # CM0341

CD63 (Extracellular region) M034 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:100
IP	1:100
WB	1:2000

Size 100 μl

Species Reactivity Hu

MW 30-60 kDa

Isotype IgG1

Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60 kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis.

References

Pols, MS & Klumperman, J (2009) Exp Cell Res. 315(9):1584.
Metzelaar, M.J. et al. (1991) J Biol Chem. 266(5):3239.
Azorsa, D.O. et al. (1991) Blood. 78(2):280.

Western blot of native human cell and tissue lyastes: A549 (lane 1), and A549 treated with PNGase F (lane 2), MDA-MB-231 (lane 3), MCF7 (lane 4), breast (lane 5), lung (lane 6), and skin (lane 7). The blot was probed with mouse monoclonal CD63 (CM0341) at 1:1000.

Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0341). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD63 (CM0341) for ELISA capture of human recombinant CD63 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Clone (M034) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line.

*For more information, see UniProt Accession P08962

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M034 mouse monoclonal antibody detects a 30-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human breast, lung, and skin tissues. This antibody does not detect denatured CD63. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: