Catalog # CM0101

CD44 (Extracellular region) M010 Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
ICC	1:100
IP	1:50
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 80 kDa

Isotype IgG2b

Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.

References

Cichy, J., et al. (2003) J Cell Biol. 161(5):839
Goodinson, S., et al. (1999) Mol Path. 52(4):189
Tsukita, S., et al. (1994) J Cell Biol. 126(2):391

Western blot analysis of human A549 (lanes 1 & 2) and human MDA-MB-231 (lanes 3 & 4) whole cell lysates under native (lanes 1 & 3) and denatured (lanes 2 & 4) conditions. The blots were probed with mouse monoclonal anti-CD44 (CM010) at 1:1000 (lanes 1-4).

Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.

Clone M010 was generated from a proprietary antigen related to the extracellular region of human CD44.from the A431 epidermoid carcinoma cell line.

*For more information, see UniProt Accession P16070

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M010 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of native and denaturing human MDA-MB-231, A549, and A431 cell lysates. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemical labeling and immunoprecipitation. In addition, the antibody labels live unfixed A549 and MeWo cells, so the antibody may be useful for isolating CD44+ cells and for CD44 functional assays.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: