Catalog # AM5621

ALDH1A1 (N-terminal region) M562 Antibody

Mouse Monoclonal

Application / Dilution

ELISA	1:2000
WB	1:1000
ICC	1:100

Size 100 μl

Species Reactivity Hu

MW 55 kDa

Isotype IgG2a

Aldehyde dehydrogenase (ALDH) superfamily is a ubiquitous group of enzymes found in all taxonomic domains. ALDH detoxifies endogenous and exogenous aldehydes, protecting cellular homeostasis and organismal functions. These enzymes are necessary for the synthesis of retinoic acid, betaine, and folate. Recent studies have reported high levels of ALDH found in cancer cells, suggesting that ALDH can act as a marker for cancer cells found in a wide variety of tissues including skin, prostate, lung, and neural tissues. Additionally, certain diseases can be identified when ALDH activity is absent. ALDH1A1 is vital for retinol synthesis and alcohol metabolism. ALDH1A1 active sites include an active cysteine residue, which catalyses the transformation of aldehydes into their respective carboxylic groups. ALDH1A1 amino acid sequence and function is highly conserved in humans and rodents.

References

Luo, Y. et al. (2012) Stem Cells. 30(10):2100.
Jackson, B. et al. (2011) Hum Genomics. 5(4):283.
Edenberg, H.J. (2007) Alcohol Res Health. 30(1):5.
Duester, G. (2000) Eur J Biochem. 267(14):4315.

Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M562 (lanes 1-6) at a dilution of 1:1,000.

Immunocytochemical labeling of ALDH1A1 in aldehyde fixed and NP-40 permeabilized human A431 cells. The cells were labeled with mouse monoclonal anti-ALDH1A1 (AM5621). The antibody was detected using goat anti-mouse DyLight® 594.

Clone M562 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human ALDH1A1.

*For more information, see UniProt Accession P00352

Mouse monoclonal purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The antibody was purified by Protein G chromatography. This antibody antibody detects a 55 kDa* protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: