Catalog # CM0481

CD155/PVR (Extracellular region) Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:100
IP	1:100
WB	1:1000

Size 100 μl

Species Reactivity Hu

MW 75 kDa

Isotype IgG1

CD155/Poliovirus receptor (PVR)/nectin-like 5 (Necl-5) is a transmembrane glycoprotein with extracellular immunoglobulin like domains, and an intracellular immunoreceptor tyrosine-based inhibitor motif (ITIM). CD155 was originally described as a mediator of poliovirus attachment to cells, but has also been implicated in adherens junction formation. CD155 binds nectin-3, and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells. CD155 is also a ligand for immunoreceptors that regulate tumor surveillance. CD155 binds DNAX-associated molecule 1 (DNAX-1), an activating receptor on natural killer cells and cytotoxic T-cells. Alternatively, CD155 may bind TIGIT immunoreceptor inducing an immunosuppressive and non-cytotoxic profile. In cancers, CD155 expression has been associated with unfavorable prognosis in colon cancer, breast cancer, lung adenocarcinoma, pancreatic cancer, melanoma, and glioblastoma. Cancer therapies have targeted CD155 interactions with TIGIT, and have used CD155 as a point of entry for recombinant oncolytic polioviruses.

References

Gao, J. (2017) Cancer Sci. 108(10):1934.
Molfetta, R. (2020) Int J Mol Sci. 21(3):922.

Western blot of human A549 lung carcinoma (lane 1), NCI-H446 small cell lung carcinoma (lane 2), NCI-H1299 lung carcinoma (lane 3), MDA-MB-231 breast carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD155 (CM0481) at 1:500.

Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-CD155 (CM0481) for ELISA capture of human recombinant CD155 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.

Clone (M048) was generated from a recombinant protein that included the extracellular region of human CD155 protein.

*For more information, see UniProt Accession P15151

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M048 mouse monoclonal antibody detects a 75 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H466, NCI-H1299, MDA-MB-231 and A431 carcinomas. The antibody detects CD155 in the membranes and cytoplasm in NCI-H446 cells after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: