Catalog # WP2001

N-WASP Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 65 kDa

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.

References

Cory, G.O. et al. (2003) Mol Cell. 11(5):1229-39.
Higgs, H.N. & Pollard, T.D. (2001) Annu Rev Biochem 70:649-676.
Baba, Y. et al. (1999) Blood 93:2003.

Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 µg/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human N-WASP. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP.

*For more information, see UniProt Accession O00401

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa* proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa* WASP protein in Jurkat cell lysate.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Kalwa, H. & Michel, T. (2011) J Biol Chem 286:2320. (WB: bovine aortic endothelial cells)
Pichot, C. et al. (2010) Cancer Res. 70:8347. (WB: human MDA-MB-231 cells)
Anitei, M. et al. (2010) Nature Cell Biol. 12:330. (WB: human HeLa cells)
Pichot, C. et al. (2009) British J Cancer. 1 10. (WB: breast cancer cell lines)
Elias, BC et al. (2015) J Cell Sci. 128(23):4293. (WB: mouse kidney duct cells)
Hartig, S.M. et al. (2009) J Cell Sci. 122:2283. (WB: rat L6 myoblasts)

This kit contains: