Catalog # WP1821

WAVE2 (Tyr-150)[conserved site], phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:200
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 78 kDa

The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as, membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

References

Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
Leng, Y. et al. (2005) PNAS 102(4):1098.
Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.
Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.

Western blot of human K562 cells treated with pervanadate (1 mM, 30 min) (lanes 1 & 3) then treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with anti-WAVE2 (Central region) (lanes 1 & 2) or anti-WAVE (Tyr-150) (lanes 3 & 4).

Phospho-WAVE (Tyr-150) peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has homology to the conserved sites in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).

*For more information, see UniProt Accession Q9Y6W5

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to phospho-WAVE (Tyr-125) and unphosphorylated WAVE (Tyr-150) peptides before affinity purification using phospho-WAVE (Tyr-150) peptide. In western blots, the antibody detects a 78 kDa* corresponding to phosphorylated WAVE2 in K562 cells treated with pervanadate and a 80 kDa band corresponding to WAVE1 in HUVEC treated with pervanadate. It also recognizes an unidentified band near 50 kDa in K562 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Kritikou, JS et al. (2016) Sci Rep. 6:30636. (WB: mouse NK cells)
Bae, H. et al. (2011) FASEB J. 25(12):4358. (WB: mouse peritoneal macrophages)

This kit contains: