Catalog # TP1781

β-Tubulin (a.a. 168-177) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:50
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms, Ck

MW 50 kDa

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

References

Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Fanarraga, M.L. et al. (1999) Eur. J. Neurosci. 11:517.
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.

Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).

Immunocytochemical labeling of β-tubulin in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-β-Tubulin (TP1781). The antibody was detected using goat anti-rabbit DyLight® 594.

βIII-Tubulin (a.a. 168-177) synthetic peptide (coupled to KLH) corresponding to amino acid residues from human βIII-Tubulin. This sequence is identical to similar regions in βI, βII, and βIII-Tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.

*For more information, see UniProt Accession Q13509

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using βIII-Tubulin (a.a. 168-177) peptide (without carrier). This antibody detects a 50 kDa* protein corresponding to the molecular mass of β-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: