Catalog # SP1371

c-Src (Tyr-215)[conserved site], phospho-specific Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 60 kDa

c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.

References

Vadlamudi, R.K. et al. (2003) FEBS Letters 543:76.
Obergfell, A. et al. (2002) J Cell Biol. 157(2):265.
Stover, D.R. et al. (1996) J Biol Chem 271(21):12481.

Western blot analysis of mouse SYF cells transformed with c-Src then left untreated (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-c-Src (Tyr-215) (lanes 1 & 2), anti-c-Src (N-terminal region) (lanes 3 & 4), and anti-c-Src (Tyr-530) (lanes 5 & 6).

c-Src (Tyr-215) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 215 of human c-Src. This sequence has homology to the conserved site in other Src family members, such as hck, fyn, csk, and yes.

*For more information, see UniProt Accession P12931

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Src (Tyr-215) peptide (without carrier). The antibody detects a 60 kDa* protein on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells that have been treated with pervanadate.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Pentassuglia, L et al. (2016) AJP Endocr Metab. 310(9):E782. (WB: rat cardiomyocytes)
Lindquist, S. et al. (2011) PLoS ONE 6(9):e23978. (Western blot)

This kit contains: