Catalog # PP4491
Paxillin (Thr-538), phospho-specific Antibody
Rabbit Polyclonal
| ELISA | 1:2000 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms, Ck, F
MW 68 kDa
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at tyrosine, threonine, and serine sites. Serine and threonine phosphorylation of paxillin occur in response to growth-factor activation, PKC activators, and fibronectins. Phosphorylation of Ser-85, Ser-178, and Thr-538 may be important sites for regulating paxillin activity. Paxillin phosphorylation of Thr-538 occurs in response to TPA-activated PKCs in vitro, and this phosphorylation may contribute to dissolution of the actin cytoskeleton and redistribution of LFA-1 integrins in vivo.
References
Ishibe, S. et al. (2004) Mol. Cell 16 :257-267.
Huang, C. et al. (2003) Nature 424:219-223.
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
Western blot analysis of human A431 cells treated with Calyculin A (100 nm) for 30 min (lanes 1-6). The blot was treated with lambda phosphatase (lanes 2, 4, & 6), then probed with rabbit polyclonal anti-Paxillin (C-terminal; PP1161) (lanes 1 & 2), anti-phospho-Paxillin (Thr-538; PP4491) (lanes 3 & 4), and mouse monoclonal anti-Paxillin (PM1071) (lanes 5 & 6).
*For more information, see UniProt Accession P49023
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
