Catalog # PP1841

Plexin B1 (C-terminal region) Antibody

Rabbit Polyclonal

Application / Dilution

ELISA	1:2000
ICC	1:400
WB	1:1000

Size 100 μl

Species Reactivity Hu, Rt, Ms

MW 300 kDa

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. In addition, Plexin B1 and B2 contain cleavage sites for subtilisin-like proprotein convertases. These cleavage sites may be important for regulating assembly of heterodimeric receptors or release of the extracellular domain. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D. Sema-4D activation of Plexin B1 recruits and activates c-Met, which can promote cell survival, migration, and angiogenesis.

References

Basile, J.R. et al. (2006) PNAS 103(24):9017.
Artigiani, S. et al. (2003) J Biol. Chem. 278(12):10094.
Tamagnone, L. et al. (1999) Cell 99:71.

Western blot of mouse liver (lane 1) and rat PC12 cells (lane 2). The blot was probed with rabbit polyclonal Plexin B1 antibody (PP1841) at a dilution of 1:1000.

Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (PP1841) and mouse monoclonal Sema-4D (SM1881) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Plexin B1. This sequence has high homology with similar regions in rat and mouse Plexin B1, and has low homology to other plexin family members.

*For more information, see UniProt Accession O43157

Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody was affinity purified using Plexin B1 (C-terminal region) peptide (without carrier). The antibody detects 300, 150, and 100 kDa* bands on SDS-PAGE immunoblots of mouse liver and rat PC12 cells. These bands correspond to the full length and proteolytically processed forms of Plexin B1. In mouse liver, this antibody detects 300 and 100 kDa forms of Plexin B1.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

Williamson, M. et al. (2016) Oncogene. 35(8):1066. (WB: human LNCaP, siRNA)
Damola, A. et al. (2013) Prostate 73(12):1326. (WB, IP: Prostate Cells, shRNA knockdown)
Soong, J & Scott, G. (2013) J Cell Sci. 126(Pt 2):688. (WB: human melanocytes)
Soong, J. et al (2012) J Invest Dermatol. 132(4):1230. (WB: human melanocytes, keratinocytes, and smooth muscle)

This kit contains: