Catalog # PM1071
Paxillin Antibody
Mouse Monoclonal
| ELISA | 1:2000 |
| ICC | 1:100 |
| IP | 1:100 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms, Ck
MW 68 kDa
Isotype IgG1
Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
References
Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Huang, C. et al. (2003) Nature 424:219-223.
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).
Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.
*For more information, see UniProt Accession P49023
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
Stone, JD et al. (2016) J Non Invasive Vasc Invest. 1:2-9. (FC: rat ATr5 smooth muscle cells)Dasgupta, SK et al. (2017) Cancer Med. 6(4):809. (WB: HMEC-1 cells)
Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. (IF: mouse NIH3T3 fibroblasts)
Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 (IF: B16-F1 cells)
Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. (WB: rat C6 and human U373 gliomas)
Liu, H. et al. (2013) BMC Cancer. 13:80. (WB: human ovarian cancer cells)
Richard, D. et al. (2008) Acta Neurophathologica 115(2):205. (Immunocytochemistry)
This kit contains:
