Catalog # EM0881

EphA2 (Extracellular region) M088 Antibody

Mouse Monoclonal

Application / Dilution

ELISA (Cap)	1:100
ICC	1:200
IP	1:100

Size 100 μl

Species Reactivity Hu

MW 125 kDa

Isotype IgG1

The Eph family of receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. Ephrin type-A receptor 2 (EphA2), also known as epithelial cell kinase (Eck), binds the ephrin A1 (EFNA1) ligand, and has roles in neuronal development and repair, as well as carcinogenesis. EphA2 receptor has an N-terminal ligand-binding domain followed by a cysteine-rich domain with an epidermal growth factor-like motif and two fibronectin type-III repeats in the extracellular region, and a sterile alpha motif (SAM), and a PDZ domain-binding motif in the intracellular region. EphA2 is expressed in many types of cancers, including breast, colon, bladder, gastric, and glioblastoma. In bladder cancers, EphA2 may be activated by progranulin leading to phosphorylation at Ser-897 and bladder tumorigenesis. EphA2 may be an important therapeutic target and biomarker for several types of cancer.

References

Neill, T. et al. (2016) J Cell Biol. 215(5):687.
Zhou, Y. & Sakurai, H. (2017) Biol Pharm Bull. 40(10):1616.

Representative Standard Curve using mouse monoclonal anti-EphA2 (EM0881) for ELISA capture of human recombinant EphA2 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.

Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (EM0881). The antibody was detected using goat anti-mouse DyLight® 594.

Clone (M088) was generated from a recombinant protein that included the extracellular region of human EphA2 protein.

*For more information, see UniProt Accession P29317

Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M088 mouse monoclonal antibody detects native human EphA2, as well as aldehyde-fixed EphA2. The antibody works for staining in immunocytochemistry, capture and detection in ELISA, and native immunoprecipitation assays.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: