Catalog # DP3671
Draxin (C-terminal region) Antibody
Rabbit Polyclonal
| ELISA | 1:2000 |
| ICC | 1:100 |
| WB | 1:1000 |
Size 100 μl
Species Reactivity Hu, Rt, Ms, Ck
MW 50 kDa
The developing nervous system assembles with the help of guidance molecules that control the trajectory of growing neurons. Axon guidance proteins such as, netrins, semaphorins, ephrins, and slits, are critical extracellular matrix cues for correctly wiring of the nervous system. Draxin is an axon guidance protein that has been observed to repel neurite outgrowth from dorsal spinal cord and cortical explants in vitro. Chick draxin consists of 349 amino acids with a putative signal peptide sequence at the N-terminal end but no membrane anchoring sequence, which suggests that draxin is a secreted protein. The ectopically expressed draxin has been shown to inhibit growth or cause misrouting of chick spinal cord commissural axons in vivo. In both chick and mouse, commissural neurons require draxin to successfully cross the midline and form the major commissures of the brain. Thus, draxin is a chemorepulsive axon guidance molecule required for the development of the spinal cord and forebrain commissures.
References
Islam, S. M. et al. (2009) Science 323(5912):388.
Western blot analysis of draxin expression in rat PC12 cells (lane 1), rat P1 brain (lane 2), adult mouse brain (lane 3), and chick E9 brain (lane 4). The blot was probed with rabbit polyclonal anti-Draxin (C-terminal region) at 1:1000.
Immunocytochemical labeling of Draxin in rat PC12 cells differentiated with NGF. The cells were probed with Draxin (C-terminal region) rabbit polyclonal antibody, then the antibody was detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (DX3675). Lower images show corresponding phase images.
*For more information, see UniProt Accession Q8NBI3
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
